|Application ||WB, E|
|Other Accession||NP_009093, 7942, 21425 (mouse), 316214 (rat)|
|Predicted||Mouse, Rat, Dog|
|Calculated MW||52865 Da|
|Other Names||Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35, TFEB, BHLHE35|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-TFEB (internal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.|
|Cellular Location||Cytoplasm. Nucleus Note=Mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). In macrophages, translocates into the nucleus upon live S.enterica infection (PubMed:27184844).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
A gene network regulating lysosomal biogenesis and function. Sardiello M, et al. Science, 2009 Jul 24. PMID 19556463.
Cathepsin-K immunoreactivity distinguishes MiTF/TFE family renal translocation carcinomas from other renal carcinomas. Martignoni G, et al. Mod Pathol, 2009 Aug. PMID 19396149.
Molecular genetics of adult ADHD: converging evidence from genome-wide association and extended pedigree linkage studies. Lesch KP, et al. J Neural Transm, 2008 Nov. PMID 18839057.
Characterization of t(6;11)(p21;q12) in a renal-cell carcinoma of an adult patient. Pecciarini L, et al. Genes Chromosomes Cancer, 2007 May. PMID 17285572.
Renal carcinomas with the t(6;11)(p21;q12): clinicopathologic features and demonstration of the specific alpha-TFEB gene fusion by immunohistochemistry, RT-PCR, and DNA PCR. Argani P, et al. Am J Surg Pathol, 2005 Feb. PMID 15644781.
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