|Application ||WB, IHC, E|
|Other Accession||NP_009093, 7942, 21425 (mouse)|
|Predicted||Mouse, Rat, Pig, Dog, Cow|
|Calculated MW||52865 Da|
|Other Names||Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35, TFEB, BHLHE35|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-TFEB (N Terminus) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.|
|Cellular Location||Cytoplasm. Nucleus. Note=Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus|
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Provided below are standard protocols that you may find useful for product applications.
A gene network regulating lysosomal biogenesis and function. Sardiello M, et al. Science, 2009 Jul 24. PMID 19556463. Cathepsin-K immunoreactivity distinguishes MiTF/TFE family renal translocation carcinomas from other renal carcinomas. Martignoni G, et al. Mod Pathol, 2009 Aug. PMID 19396149. Molecular genetics of adult ADHD: converging evidence from genome-wide association and extended pedigree linkage studies. Lesch KP, et al. J Neural Transm, 2008 Nov. PMID 18839057. Characterization of t(6;11)(p21;q12) in a renal-cell carcinoma of an adult patient. Pecciarini L, et al. Genes Chromosomes Cancer, 2007 May. PMID 17285572. Renal carcinomas with the t(6;11)(p21;q12): clinicopathologic features and demonstration of the specific alpha-TFEB gene fusion by immunohistochemistry, RT-PCR, and DNA PCR. Argani P, et al. Am J Surg Pathol, 2005 Feb. PMID 15644781.
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