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Goat Anti-TIM50 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-TIM50 Antibody AF2090a
    AF2090a staining (0.5 µg/ml) of Human Heart lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q3ZCQ8
Other Accession NP_001001563, 92609
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 39646 Da
Additional Information
Gene ID 92609
Other Names Mitochondrial import inner membrane translocase subunit TIM50, TIMM50, TIM50
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-TIM50 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TIMM50
Synonyms TIM50
Function Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein
Tissue Location Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level)
Research Areas
Citations (0)

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Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.
A human protein-protein interaction network: a resource for annotating the proteome. Stelzl U, et al. Cell, 2005 Sep 23. PMID 16169070.
Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis. Xu H, et al. BMC Cell Biol, 2005 Jul 11. PMID 16008839.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death. Guo Y, et al. J Biol Chem, 2004 Jun 4. PMID 15044455.

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$ 325.00
Cat# AF2090a
(40 western blots)
Availability: 7-10 days
Bulk Size
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