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Goat Anti-VPS28 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-VPS28 Antibody AF2151a
    AF2151a staining (1 µg/ml) of Human Testis lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • IHC - Goat Anti-VPS28 Antibody AF2151a
    AF2151a (10 µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UK41
Other Accession NP_057292, 51160, 66914 (mouse)
Reactivity Human
Predicted Mouse, Rat, Pig, Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 25425 Da
Additional Information
Gene ID 51160
Other Names Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, VPS28
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-VPS28 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VPS28
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
Cellular Location Cell membrane. Late endosome membrane; Peripheral membrane protein
Research Areas
Citations (0)

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This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.


Budding of filamentous and non-filamentous influenza A virus occurs via a VPS4 and VPS28-independent pathway. Bruce EA, et al. Virology, 2009 Aug 1. PMID 19524996.
An empirical framework for binary interactome mapping. Venkatesan K, et al. Nat Methods, 2009 Jan. PMID 19060904.
Regulation of Tsg101 expression by the steadiness box: a role of Tsg101-associated ligase. McDonald B, et al. Mol Biol Cell, 2008 Feb. PMID 18077552.
Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.
A human protein-protein interaction network: a resource for annotating the proteome. Stelzl U, et al. Cell, 2005 Sep 23. PMID 16169070.

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$ 325.00
Cat# AF2151a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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