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Goat Anti-XRCC4-like factor / NHEJ1 Antibody

Peptide-affinity purified goat antibody

  • WB - Goat Anti-XRCC4-like factor / NHEJ1 Antibody AF2166a
    AF2166a (0.1 µg/ml) staining of Human Thyroid lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • IHC - Goat Anti-XRCC4-like factor / NHEJ1 Antibody AF2166a
    AF2166a (3.8 µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H9Q4
Other Accession NP_079058, 79840
Reactivity Human
Predicted Dog
Host Goat
Clonality Polyclonal
Concentration 100ug/200ul
Isotype IgG
Calculated MW 33337 Da
Additional Information
Gene ID 79840
Other Names Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor, NHEJ1, XLF
Format 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-XRCC4-like factor / NHEJ1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NHEJ1
Synonyms XLF
Function DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
Cellular Location Nucleus
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.


Variation within DNA repair pathway genes and risk of multiple sclerosis. Briggs FB, et al. Am J Epidemiol, 2010 Jul 15. PMID 20522537.
A genome-wide association study in 19 633 Japanese subjects identified LHX3-QSOX2 and IGF1 as adult height loci. Okada Y, et al. Hum Mol Genet, 2010 Jun 1. PMID 20189936.
Characterization of a natural mutator variant of human DNA polymerase lambda which promotes chromosomal instability by compromising NHEJ. Terrados G, et al. PLoS One, 2009 Oct 6. PMID 19806195.
Novel susceptibility loci for second primary tumors/recurrence in head and neck cancer patients: large-scale evaluation of genetic variants. Wu X, et al. Cancer Prev Res (Phila), 2009 Jul. PMID 19584075.
Requirement for XLF/Cernunnos in alignment-based gap filling by DNA polymerases lambda and mu for nonhomologous end joining in human whole-cell extracts. Akopiants K, et al. Nucleic Acids Res, 2009 Jul. PMID 19420065.

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$ 325.00
Cat# AF2166a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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