|Application ||WB, IHC, E|
|Other Accession||NP_542409, 140739, 67921 (mouse), 363284 (rat)|
|Predicted||Rat, Dog, Cow|
|Calculated MW||21077 Da|
|Other Names||NEDD8-conjugating enzyme UBE2F, 6.3.2.-, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, Ubiquitin-conjugating enzyme E2 F, UBE2F, NCE2|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-NCE2 / UBE2F Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.|
|Tissue Location||Widely expressed (at protein level).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Huang DT, et al. Mol Cell, 2009 Feb 27. PMID 19250909.
Generation and annotation of the DNA sequences of human chromosomes 2 and 4. Hillier LW, et al. Nature, 2005 Apr 7. PMID 15815621.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.
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