|Application ||WB, E|
|Other Accession||NP_055726, 22848, 269774 (mouse), 500244 (rat)|
|Predicted||Mouse, Rat, Cow|
|Calculated MW||103885 Da|
|Other Names||AP2-associated protein kinase 1, 126.96.36.199, Adaptor-associated kinase 1, AAK1, KIAA1048|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-AAK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.|
|Cellular Location||Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity).|
|Tissue Location||Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain|
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Provided below are standard protocols that you may find useful for product applications.
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Genomewide association study for onset age in Parkinson disease. Latourelle JC, et al. BMC Med Genet, 2009 Sep 22. PMID 19772629.
Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.
A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Henderson DM, et al. Mol Biol Cell, 2007 Jul. PMID 17494869.
Endocytic Ark/Prk kinases play a critical role in adriamycin resistance in both yeast and mammalian cells. Takahashi T, et al. Cancer Res, 2006 Dec 15. PMID 17178891.
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