|Application ||WB, E|
|Other Accession||NP_783297, 7082|
|Calculated MW||195459 Da|
|Other Names||Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1|
|Format||0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Goat Anti-ZO-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.|
|Cellular Location||Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction Cell junction, gap junction. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells|
|Tissue Location||The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.
Diesel exhaust particles modulate vascular endothelial cell permeability: implication of ZO-1 expression. Li R, et al. Toxicol Lett, 2010 Sep 1. PMID 20576493.
ZO-1 is involved in trophoblastic cell differentiation in human placenta. Pidoux G, et al. Am J Physiol Cell Physiol, 2010 Jun. PMID 20200207.
Insights into regulated ligand binding sites from the structure of ZO-1 Src homology 3-guanylate kinase module. Lye MF, et al. J Biol Chem, 2010 Apr 30. PMID 20200156.
Hypercholesterolaemic serum increases the permeability of endothelial cells through zonula occludens-1 with phosphatidylinositol 3-kinase signaling pathway. Bian C, et al. J Biomed Biotechnol, 2009. PMID 20150971.
Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease. Ohira M, et al. Int J Mol Med, 2009 Dec. PMID 19885626.
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