|Application ||IHC, E|
|Other Accession||NP_001009881.1, NP_056084.1, NP_001009882.1, 23318|
|Predicted||Mouse, Rat, Dog, Cow|
|Calculated MW||185166 Da|
|Other Names||Terminal uridylyltransferase 4, TUTase 4, 22.214.171.124, Zinc finger CCHC domain-containing protein 11, ZCCHC11, KIAA0191, TUT4|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZCCHC11 / PAPD3 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:25480299). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity). Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A. Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (By similarity). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.|
|Cellular Location||Nucleus. Cytoplasm Note=Mainly cytoplasmic (PubMed:19703396, PubMed:25480299) Translocates into the cytoplasm following treatment of the cell with LPS (PubMed:16643855)|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize all 3 reported isoforms (as represented by NP_001009881.1; NP_056084.1; NP_001009882.1)
A novel Zinc finger protein, ZCCHC11, interacts with TIFA and modulates TLR signaling. Minoda Y, Saeki K, Aki D, Takaki H, Sanada T, Koga K, Kobayashi T, Takaesu G, Yoshimura A. Biochem Biophys Res Commun. 2006 Jun 9;344(3):1023-30.; Erratum
in: Biochem Biophys Res Commun. 2007 Feb 23;353(4):1121. PMID: 16643855
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