|Application ||IHC, E|
|Other Accession||NP_001009881.1, NP_056084.1, NP_001009882.1, 23318|
|Predicted||Mouse, Rat, Dog, Cow|
|Calculated MW||185166 Da|
|Other Names||Terminal uridylyltransferase 4, TUTase 4, 220.127.116.11, Zinc finger CCHC domain-containing protein 11, ZCCHC11, KIAA0191, TUT4|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZCCHC11 / PAPD3 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre- let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation.|
|Cellular Location||Nucleus. Cytoplasm. Note=Translocates into the cytoplasm following treatment of the cell with LPS|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize all 3 reported isoforms (as represented by NP_001009881.1; NP_056084.1; NP_001009882.1)
A novel Zinc finger protein, ZCCHC11, interacts with TIFA and modulates TLR signaling. Minoda Y, Saeki K, Aki D, Takaki H, Sanada T, Koga K, Kobayashi T, Takaesu G, Yoshimura A. Biochem Biophys Res Commun. 2006 Jun 9;344(3):1023-30.; Erratum
in: Biochem Biophys Res Commun. 2007 Feb 23;353(4):1121. PMID: 16643855
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