|Application ||IHC, E|
|Other Accession||NP_001009881.1, NP_056084.1, NP_001009882.1, 23318|
|Predicted||Mouse, Rat, Dog, Cow|
|Calculated MW||185166 Da|
|Other Names||Terminal uridylyltransferase 4, TUTase 4, 126.96.36.199, Zinc finger CCHC domain-containing protein 11, ZCCHC11, KIAA0191, TUT4|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZCCHC11 / PAPD3 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity). Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (PubMed:19703396). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-fnctional pre-miRNA species (PubMed:25979828). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP (PubMed:16643855). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:25979828, PubMed:25480299, PubMed:16643855, PubMed:19703396, PubMed:18172165) (By similarity).|
|Cellular Location||Nucleus. Cytoplasm Note=Mainly cytoplasmic (PubMed:19703396, PubMed:25480299) Translocates into the cytoplasm following treatment of the cell with LPS (PubMed:16643855)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize all 3 reported isoforms (as represented by NP_001009881.1; NP_056084.1; NP_001009882.1)
A novel Zinc finger protein, ZCCHC11, interacts with TIFA and modulates TLR signaling. Minoda Y, Saeki K, Aki D, Takaki H, Sanada T, Koga K, Kobayashi T, Takaesu G, Yoshimura A. Biochem Biophys Res Commun. 2006 Jun 9;344(3):1023-30.; Erratum
in: Biochem Biophys Res Commun. 2007 Feb 23;353(4):1121. PMID: 16643855
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.