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FEM1A / APRAP Antibody (C-Term)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9BSK4 |
| Other Accession | NP_061178.1, 55527 |
| Reactivity | Human |
| Predicted | Dog, Cow |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/ml |
| Isotype | IgG |
| Calculated MW | 73639 Da |
| Gene ID | 55527 |
|---|---|
| Other Names | Protein fem-1 homolog A, FEM1a, FEM1-alpha, Prostaglandin E receptor 4-associated protein, FEM1A, EPRAP |
| Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | FEM1A / APRAP Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | FEM1A {ECO:0000303|PubMed:16254458, ECO:0000312|HGNC:HGNC:16934} |
|---|---|
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:33398168, PubMed:33398170). The C- degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:29779948, PubMed:33398168, PubMed:33398170). The CRL2(FEM1A) complex specifically recognizes proteins with an arginine at the C-terminus: recognizes and binds proteins ending with -Lys/Arg-Xaa-Arg and -Lys/Arg-Xaa-Xaa-Arg C- degrons, such as SIL1 or OR51B2, leading to their ubiquitination and degradation (PubMed:33398168, PubMed:33398170). Promotes ubiquitination and degradation of SLBP (PubMed:28118078). Involved in PGE2-EP4- mediated inhibition of inflammation of macrophages via interaction with NFKB1 and PTGER4 (By similarity). Promotes inflammation in brain microglia through MAP2K4/MKK4-mediated signaling (By similarity). |
| Cellular Location | Mitochondrion. Cytoplasm |
| Tissue Location | Present in macrophages derived from peripheral blood monocytes. Also present in atheromata (at protein level) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
References
Prostaglandin E receptor type 4-associated protein interacts directly
with NF-kappaB1 and attenuates macrophage activation. Minami M, Shimizu K, Okamoto Y, Folco E, Ilasaca ML, Feinberg MW, Aikawa M, Libby P. J Biol Chem. 2008 Apr 11;283(15):9692-703. PMID: 18270204
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