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BAG5 (N Terminus) Antibody (N-Term)

Peptide-affinity purified goat antibody

     
  • WB - BAG5 (N Terminus) Antibody (N-Term) AF2266a
    AF2266a (2 µg/ml) staining of nuclear HeLa lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UL15
Other Accession NP_001015049.1, NP_004864.1, NP_001015048.1, 9529
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 51200 Da
Additional Information
Gene ID 9529
Other Names BAG family molecular chaperone regulator 5, BAG-5, Bcl-2-associated athanogene 5, BAG5, KIAA0873
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBAG5 (N Terminus) Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BAG5
Synonyms KIAA0873
Function Co-chaperone for HSP/HSP70 proteins. It functions as a nucleotide-exchange factor promoting the release of ADP from HSP70, thereby activating HSP70-mediated protein refolding (PubMed:20223214). Has an essential role in maintaining proteostasis at junctional membrane complexes (JMC), where it may function as a scaffold between the HSPA8 chaperone and JMC proteins enabling correct, HSPA8-dependent JMC protein folding (By similarity). Inhibits both auto-ubiquitination of PRKN and ubiquitination of target proteins by PRKN (By similarity).
Cellular Location Note=In cardiomyocytes, localized at specialized membrane contact sites between T-tubules and the sarcoplasmic reticulum, known as junctional membrane complexes {ECO:0000250|UniProtKB:Q8CI32}
Tissue Location Expressed in the heart.
Research Areas
Citations (0)
citation

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Background

Variants (NP_004864.1; NP_001015048.1) encode the same isoform.

References

An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators. Takayama S, Xie Z, Reed JC. J Biol Chem. 1999 Jan 8;274(2):781-6. PMID: 9873016

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$ 341.00
Cat# AF2266a
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