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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Goat Antibodies   >   Centromere protein F / mitosin Antibody (C-Term)   

Centromere protein F / mitosin Antibody (C-Term)

Peptide-affinity purified goat antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49454
Other Accession AAA82889.1, AAA82935.1, AAA86889.1, 1063
Predicted Human, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 367764 Da
Additional Information
Gene ID 1063
Other Names Centromere protein F, CENP-F, AH antigen, Kinetochore protein CENPF, Mitosin, CENPF
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCentromere protein F / mitosin Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
Cellular Location Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm By telophase, is concentrated within the intracellular bridge at either side of the mid-body
Research Areas
Citations (0)

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The immunizing peptide shows one mismatch with the corresponding sequence of the NCBI-curated isoform NP_057427.3.


CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis. Liao H, Winkfein RJ, Mack G, Rattner JB, Yen TJ. J Cell Biol. 1995 Aug;130(3):507-18. PMID: 7542657

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$ 325.00
Cat# AF2279a
(40 western blots)
Availability: 7-10 days
Bulk Size
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