|Application ||IHC, E|
|Other Accession||NP_071349.3, 63893, 217342 (mouse)|
|Predicted||Mouse, Rat, Pig, Dog, Cow|
|Calculated MW||141293 Da|
|Other Names||E2/E3 hybrid ubiquitin-protein ligase UBE2O, 6.3.2.-, 188.8.131.52, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-conjugating enzyme E2-230K, Ubiquitin-protein ligase O, UBE2O, KIAA1734|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||E2-230K / UBE2O Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin- associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly (PubMed:23452853).|
|Cellular Location||Cytoplasm. Nucleus. Note=Mainly localizes to the cytoplasm|
|Tissue Location||Predominantly expressed in skeletal muscle and heart.|
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Provided below are standard protocols that you may find useful for product applications.
Identification, tissue expression, and chromosomal position of a novel gene encoding human ubiquitin-conjugating enzyme E2-230k. Yokota T, Nagai H, Harada H, Mine N, Terada Y, Fujiwara H, Yabe A, Miyazaki K, Emi M. Gene. 2001 Apr 4;267(1):95-100. PMID: 11311559
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