|Other Accession||NP_001717.2, NP_997072.1, 676, 114642 (mouse)|
|Predicted||Human, Mouse, Rat, Dog, Cow|
|Calculated MW||107954 Da|
|Other Names||Bromodomain testis-specific protein, Cancer/testis antigen 9, CT9, RING3-like protein, BRDT|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BRD6 / BRDT Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.|
|Cellular Location||Nucleus Note=Detected on chromatin|
|Tissue Location||Testis-specific. A 3-fold higher expression is seen in adult testis than in embryo testis. Expression seems to be correlated with histone H4 hyperacetylation during the haploid phase of spermatogenesis (spermiogenesis). No expression, or very low expression is seen in patients' testes with abnormal spermatogenesis. Expressed in cancers such as non-small cell lung cancer and squamous cell carcinomas of the head and neck as well as of esophagus, but not in melanoma or in cancers of the colon, breast, kidney and bladder.|
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Provided below are standard protocols that you may find useful for product applications.
Variants (NP_001717.2; NP_997072.1) encode the same protein.
Identification and characterization of BRDT: A testis-specific gene related to the bromodomain genes RING3 and Drosophila fsh. Jones MH, Numata M, Shimane M. Genomics. 1997 Nov 1;45(3):529-34. PMID: 9367677
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