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ARIH2 / TRIAD1 Antibody (C-Term)

Peptide-affinity purified goat antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IP, E
Primary Accession O95376
Other Accession NP_006312.1, 10425, 23807 (mouse)
Reactivity Human, Mouse
Predicted Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 57819 Da
Additional Information
Gene ID 10425
Other Names E3 ubiquitin-protein ligase ARIH2, ARI-2, Protein ariadne-2 homolog, 6.3.2.-, Triad1 protein, ARIH2, ARI2, TRIAD1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARIH2 / TRIAD1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARIH2
Synonyms ARI2, TRIAD1 {ECO:0000303|PubMed:1611831
Function E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin- conjugating enzyme E2 UBE2L3 (PubMed:16118314, PubMed:17646546, PubMed:19340006, PubMed:24076655). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity). The initial ubiquitin is then elongated (By similarity). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex (PubMed:24076655). Mediates 'Lys-6', 'Lys-48'-and 'Lys- 63'-linked polyubiquitination (PubMed:16118314, PubMed:17646546, PubMed:19340006). May play a role in myelopoiesis (PubMed:19340006).
Cellular Location Nucleus. Cytoplasm
Tissue Location Widely expressed with higher expression in granulocytes.
Research Areas
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References

TRIADs: a new class of proteins with a novel cysteine-rich signature. van der Reijden BA, Erpelinck-Verschueren CA, Lowenberg B, Jansen JH. Protein Sci. 1999 Jul;8(7):1557-61. PMID: 10422847

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$ 335.00
Cat# AF2311a
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