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Neuro-d4 / DPF1 Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • WB - Neuro-d4 / DPF1 Antibody (C-Term) AF2313a
    AF2313a(1 µg/ml) staining of Human Brain (Cerebellum) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q92782
Other Accession NP_001128627.1, NP_004638.2, NP_001128628.1, 8193, 29861 (mouse), 50545 (rat)
Reactivity Human
Predicted Mouse, Rat, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 42502 Da
Additional Information
Gene ID 8193
Other Names Zinc finger protein neuro-d4, BRG1-associated factor 45B, BAF45B, D4, zinc and double PHD fingers family 1, DPF1, BAF45B, NEUD4
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeuro-d4 / DPF1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DPF1
Synonyms BAF45B, NEUD4
Function May have an important role in developing neurons by participating in regulation of cell survival, possibly as a neurospecific transcription factor. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
Cellular Location Cytoplasm. Nucleus
Research Areas
Citations (0)

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Background

This antibody is expected to recognize all three reported isoforms (NP_001128627.1; NP_004638.2; NP_001128628.1).

References

The d4 gene family in the human genome. Chestkov AV, Baka ID, Kost MV, Georgiev GP, Buchman VL. Genomics. 1996 Aug 15;36(1):174-7. PMID: 8812431

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$ 325.00
Cat# AF2313a
Size:
Quantity:
(40 western blots)
Availability: 7-10 days
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