|Application ||WB, IF, IP, ICC, E|
|Other Accession||NP_056133.2, NP_001124427.1, 23370|
|Reactivity||Human, Dog, Medaka|
|Calculated MW||130780 Da|
|Other Names||Rho guanine nucleotide exchange factor 18, 114 kDa Rho-specific guanine nucleotide exchange factor, p114-Rho-GEF, p114RhoGEF, Septin-associated RhoGEF, SA-RhoGEF, ARHGEF18, KIAA0521|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||P114-RHO-GEF / ARHGEF18 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells (PubMed:22006950).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Apical cell membrane|
|Tissue Location||Expressed in all tissues tested with highest expression in kidney and pancreas. Weakly or not expressed in liver, skeletal muscle and testis.|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
Identification and characterization of a novel Rho-specific guanine nucleotide exchange factor. Blomquist A, Schworer G, Schablowski H, Psoma A, Lehnen M, Jakobs KH, Rumenapp U. Biochem J. 2000 Dec 1;352 Pt 2:319-25. PMID: 11085924
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