|Application ||IHC, E|
|Other Accession||NP_060275.2, 54922|
|Calculated MW||103457 Da|
|Other Names||Ras-interacting protein 1, Rain, RASIP1|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RASIP1 / RAIN Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the proper formation of vascular structures that develop via both vasculogenesis and angiogenesis. Acts as a critical and vascular-specific regulator of GTPase signaling, cell architecture, and adhesion, which is essential for endothelial cell morphogenesis and blood vessel tubulogenesis. Regulates the activity of Rho GTPases in part by recruiting ARHGAP29 and suppressing RhoA signaling and dampening ROCK and MYH9 activities in endothelial cells (By similarity). May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS- mediated transformation. Negative regulator of amino acid starvation-induced autophagy.|
|Cellular Location||Cytoplasm, perinuclear region. Golgi apparatus, Golgi stack. Note=Associated with perinuclear vesicles. Is recruited to Golgi stacks by activated HRAS|
|Tissue Location||Highly expressed in heart. Detected at lower levels in placenta and pancreas.|
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Provided below are standard protocols that you may find useful for product applications.
Identification and Characterization of Rain, a Novel Ras-interacting Protein with a Unique Subcellular Localization Natalia Y. Mitin, Melissa B. Ramocki, Alfred J. Zullo, Channing J. Der, Stephen F. Konieczny, and Elizabeth J. Taparowsky J. Biol. Chem. 2004;279 22353-22361 PMID: 15031288
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