|Other Accession||NP_060621.3, 55183|
|Calculated MW||274466 Da|
|Other Names||Telomere-associated protein RIF1, Rap1-interacting factor 1 homolog, RIF1|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RIF1 (aa 2076-2089) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S- phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints.|
|Cellular Location||Nucleus. Chromosome, telomere. Cytoplasm, cytoskeleton, spindle. Note=Exhibits ATM- and TP53BP1-dependent localization to uncapped or aberrant telomeres and to DNA double strand breaks (DSBs). Does not associate with normal telomere structures. Localizes to microtubules of the midzone of the mitotic spindle during anaphase, and to condensed chromosomes in telophase|
|Tissue Location||Highly expressed in testis.|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognise an epitope corresponding to aa 2076-2089 of human RIF1 protein.
Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Silverman J, Takai H, Buonomo SB, Eisenhaber F, de Lange T. Genes Dev. 2004 Sep 1;18(17):2108-19. PMID: 15342490
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