LRP6 Antibody (internal region)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC, FC, Pep-ELISA |
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Primary Accession | O75581 |
Other Accession | NP_002327.2, 4040, 16974 (mouse) |
Reactivity | Human |
Predicted | Mouse, Rat, Dog |
Host | Goat |
Clonality | Polyclonal |
Concentration | 0.5 mg/ml |
Isotype | IgG |
Calculated MW | 180429 Da |
Gene ID | 4040 |
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Other Names | Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6 |
Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | LRP6 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | LRP6 |
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Function | Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalosomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity). |
Cellular Location | Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Membrane raft. Note=On Wnt signaling, undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location. Released from the endoplasmic reticulum on palmitoylation Mono-ubiquitination retains it in the endoplasmic reticulum in the absence of palmitoylation. On Wnt signaling, phosphorylated, aggregates and colocalizes with AXIN1 and GSK3B at the plasma membrane in LRP6- signalosomes. Chaperoned to the plasma membrane by MESD (By similarity). |
Tissue Location | Widely coexpressed with LRP5 during embryogenesis and in adult tissues |
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.
References
LDL-receptor-related proteins in Wnt signal transduction. Tamai K, Semenov M, Kato Y, Spokony R, Liu C, Katsuyama Y, Hess F, Saint-Jeannet JP, He X. Nature. 2000 Sep 28;407(6803):530-5. PMID: 11029007
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