|Application ||WB, E|
|Other Accession||NP_004776.3, NP_001239005.1, 9351|
|Calculated MW||37414 Da|
|Other Names||Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHERF-2, NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1, SIP-1, Sodium-hydrogen exchanger regulatory factor 2, Solute carrier family 9 isoform A3 regulatory factor 2, Tyrosine kinase activator protein 1, TKA-1, SLC9A3R2, NHERF2|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NHERF2 (isoform b, e) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3 (PubMed:18829453). May also act as scaffold protein in the nucleus.|
|Cellular Location||Endomembrane system; Peripheral membrane protein. Nucleus. Apical cell membrane Note=Localizes with EZR and PODXL at the apical cell membrane of glomerular epithelium cells and the sides of the food processes (By similarity). Nuclear, in a punctate pattern.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize isoforms b and e (NP_004776.3 and NP_001239005.1) only.
cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein. Cha B, Kim JH, Hut H, Hogema BM, Nadarja J, Zizak M, Cavet M, Lee-Kwon W, Lohmann SM, Smolenski A, Tse CM, Yun C, de Jonge HR, Donowitz M. J Biol Chem. 2005 Apr 29;280(17):16642-50. Epub 2005 Feb 18. PMID: 15722341
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