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SIRT3 Antibody (C-Term)

Peptide-affinity purified goat antibody

  • WB - SIRT3 Antibody (C-Term) AF2462a
    AF2462a (0.2 µg/ml) staining of Human Liver lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • WB - SIRT3 Antibody (C-Term) AF2462a
    HEK293 overexpressing SIRT3 (RC200190) and probed with AF2462a (mock transfection in first lane), tested by Origene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NTG7
Other Accession NP_036371.1, NP_001017524.1, 23410
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 43573 Da
Additional Information
Gene ID 23410
Other Names NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3, 3.5.1.-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, SIRT3, SIR2L3
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIRT3 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIRT3
Synonyms SIR2L3
Function NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Cellular Location Mitochondrion matrix
Tissue Location Widely expressed.
Research Areas
Citations (0)

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This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).


SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes. Shi T, Wang F, Stieren E, Tong Q. J Biol Chem. 2005 Jan 14; [Epub ahead of print] PMID: 15653680

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$ 325.00
Cat# AF2462a
(40 western blots)
Availability: 7-10 days
Bulk Size
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