GLI2 Antibody (internal region)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC, E |
---|---|
Primary Accession | P10070 |
Other Accession | NP_005261.2, 2736 |
Predicted | Human, Mouse |
Host | Goat |
Clonality | Polyclonal |
Concentration | 0.5 mg/ml |
Isotype | IgG |
Calculated MW | 167783 Da |
Gene ID | 2736 |
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Other Names | Zinc finger protein GLI2, GLI family zinc finger protein 2 {ECO:0000312|HGNC:HGNC:4318}, Tax helper protein, GLI2 (HGNC:4318) |
Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | GLI2 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GLI2 (HGNC:4318) |
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Function | Functions as a transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992, PubMed:26565916). Functions as a transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856). |
Cellular Location | Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q0VGT2}. Cell projection, cilium {ECO:0000250|UniProtKB:Q0VGT2}. Note=STK36 promotes translocation to the nucleus. In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus {ECO:0000250|UniProtKB:Q0VGT2} [Isoform 2]: Nucleus |
Tissue Location | Expressed in breast cancers (at protein level) (PubMed:26565916). Isoform 1 and isoform 4 are expressed in HTLV-1- infected T-cell lines (at protein level) (PubMed:9557682). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines (PubMed:9557682). Isoform 3 and isoform 4 are weakly expressed in HTLV- 1-infected T-cell lines (PubMed:9557682). |
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)
References
Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function. Haycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK. PLoS Genet. 2005 Oct;1(4):e53. Epub 2005 Oct 28. PMID: 16254602
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