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Semaphorin 5A Antibody (internal region)

Peptide-affinity purified goat antibody

  • IHC - Semaphorin 5A Antibody (internal region) AF2525a
    AF2525a (4 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13591
Other Accession NP_003957.1, 9037, 20356 (mouse), 310207 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 120615 Da
Additional Information
Gene ID 9037
Other Names Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSemaphorin 5A Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms SEMAF
Function Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (By similarity). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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The immunizing peptide corresponds to a part of the extracellular domain.


High-resolution whole-genome association study of Parkinson disease Maraganore DM, de Andrade M, Lesnick TG, Strain KJ, Farrer MJ, Rocca WA, Pant PV, Frazer KA, Cox DR, Ballinger DG. Am J Hum Genet. 2005 Nov;77(5):685-93. Epub 2005 Sep 9. PMID: 16252231

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$ 325.00
Cat# AF2525a
(40 western blots)
Availability: 7-10 days
Bulk Size
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