|Application ||WB, E|
|Other Accession||NP_054878.5, 29072, 235626 (mouse)|
|Predicted||Human, Pig, Dog|
|Calculated MW||287597 Da|
|Other Names||Histone-lysine N-methyltransferase SETD2, 220.127.116.11, HIF-1, Huntingtin yeast partner B, Huntingtin-interacting protein 1, HIP-1, Huntingtin-interacting protein B, Lysine N-methyltransferase 3A, SET domain-containing protein 2, hSET2, p231HBP, SETD2, HIF1, HYPB, KIAA1732, KMT3A, SET2|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HYPB / SETD2 (internal region) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HIF1, HYPB, KIAA1732, KMT3A, SET2|
|Function||Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. Trimethylates 'Lys-40' of alpha-tubulins such as TUBA1B (alpha-TubK40me3) (PubMed:27518565). Alpha- TubK40me3 is required for normal mitosis and cytokinesis and is proposed to be a specific tag in cytoskeletal remodeling (PubMed:27518565).|
|Cellular Location||Nucleus. Chromosome|
|Tissue Location||Ubiquitously expressed.|
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Identification and characterization of a novel human histone H3 lysine 36-specific methyltransferase. Sun XJ, Wei J, Wu XY, Hu M, Wang L, Wang HH, Zhang QH, Chen SJ, Huang QH, Chen Z. J Biol Chem. 2005 Oct 21;280(42):35261-71. Epub 2005 Aug 22. PMID: 16118227
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