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TRPV3 (aa762-773) Antibody (internal region, near C-Term)

Peptide-affinity purified goat antibody

     
  • WB - TRPV3 (aa762-773) Antibody (internal region, near C-Term) AF2811a
    HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRPV3 with DYKDDDDK tag probed with AF2811a (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/10000) in lane C. Mock-transfected HEK293 probed with AF2811a (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8NET8
Other Accession NP_659505.1, 162514, 246788 (mouse), 497948 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 90636 Da
Additional Information
Gene ID 162514
Other Names Transient receptor potential cation channel subfamily V member 3, TrpV3, Vanilloid receptor-like 3, VRL-3, TRPV3
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRPV3 (aa762-773) Antibody (internal region, near C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRPV3
Function Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen).
Cellular Location Membrane; Multi-pass membrane protein.
Tissue Location Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level) Expressed in the keratinocyte layers of the outer root sheath and, to lesser extent, to the matrix of the hair follicles (at protein level)
Research Areas
Citations (0)
citation

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References

TRPV3 is a calcium-permeable temperature-sensitive cation channel. Xu H, Ramsey IS, Kotecha SA, Moran MM, Chong JA, Lawson D, Ge P, Lilly J, Silos-Santiago I, Xie Y, DiStefano PS, Curtis R, Clapham DE. Nature. 2002 Jul 11;418(6894):181-6. Epub 2002 Jun 23 PMID: 12077604

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Discontinued
Cat# AF2811a
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