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CMA1 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - CMA1 Antibody (internal region) AF2833a
    HEK293 lysate (10ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with AF2833a (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with AF2833a(1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
  • IHC - CMA1 Antibody (internal region) AF2833a
    AF2833a (2µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23946
Other Accession NP_001827.1, 1215
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 27325 Da
Additional Information
Gene ID 1215
Other Names Chymase,, Alpha-chymase, Mast cell protease I, CMA1, CYH, CYM
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCMA1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CMA1
Synonyms CYH, CYM
Function Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Cellular Location Secreted. Cytoplasmic granule. Note=Mast cell granules
Tissue Location Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions
Research Areas
Citations (0)

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Activated mast cells induce endothelial cell apoptosis by a combined action of chymase and tumor necrosis factor-alpha Heikkilä HM, Lätti S, Leskinen MJ, Hakala JK, Kovanen PT, Lindstedt KA Arterioscler Thromb Vasc Biol. 2008 Feb;28(2):309-14. Epub 2007 Dec 13 PMID: 18079408

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$ 325.00
Cat# AF2833a
(40 western blots)
Availability: 7-10 days
Bulk Size
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