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SFRP4 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - SFRP4  Antibody (internal region) AF2890a
    AF2890a (0.5 µg/ml) staining of Human Uterus lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • IHC - SFRP4  Antibody (internal region) AF2890a
    AF2890a (2µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6FHJ7
Other Accession NP_003005.2, 6424, 20379 (mouse), 89803 (rat)
Reactivity Human
Predicted Mouse, Rat, Pig, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 39827 Da
Additional Information
Gene ID 6424
Other Names Secreted frizzled-related protein 4, sFRP-4, Frizzled protein, human endometrium, FrpHE, SFRP4, FRPHE
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSFRP4 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SFRP4
Synonyms FRPHE
Function Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
Cellular Location Secreted. Note=Cytoplasmic in ovarian tumor cells
Tissue Location Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level)
Research Areas
Citations (0)

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Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R. Genes Dev. 2007 Dec 1;21(23):3110-22. PMID: 18056424

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$ 325.00
Cat# AF2890a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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