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SPARTIN Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - SPARTIN Antibody (internal region) AF2902a
    AF2902a (2 µg/ml) staining of HeLa lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IF - SPARTIN Antibody (internal region) AF2902a
    AF2902a (2 µg/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.
    detail
  • WB - SPARTIN Antibody (internal region) AF2902a
    EB09136 (2µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IF - SPARTIN Antibody (internal region) AF2902a
    EB09136 (2µg/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q8N0X7
Other Accession NP_055902.1, 23111
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 72833 Da
Additional Information
Gene ID 23111
Other Names Spartin, Spastic paraplegia 20 protein, Trans-activated by hepatitis C virus core protein 1, SPG20, KIAA0610, TAHCCP1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPARTIN Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPART (HGNC:18514)
Function May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis (PubMed:20719964).
Cellular Location Cytoplasm. Midbody. Note=Transiently associated with endosomes (PubMed:19580544). Colocalized with IST1 to the ends of Flemming bodies during cytokinesis (PubMed:20719964)
Tissue Location Ubiquitously expressed, with highest levels of expression detected in adipose tissue
Research Areas
Citations (0)
citation

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References

The hereditary spastic paraplegia protein spartin localises to mitochondria Lu J, Rashid F, Byrne PC. J Neurochem. 2006 Sep;98(6):1908-19. PMID: 16945107

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$ 341.00
Cat# AF2902a
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