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AKAP9 / YOTIAO Antibody (N-Term)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| E |
|---|---|
| Primary Accession | Q99996 |
| Other Accession | NP_005742.4, NP_671714.1, 10142 |
| Predicted | Human, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/ml |
| Isotype | IgG |
| Calculated MW | 452987 Da |
| Gene ID | 10142 |
|---|---|
| Other Names | A-kinase anchor protein 9, AKAP-9, A-kinase anchor protein 350 kDa, AKAP 350, hgAKAP 350, A-kinase anchor protein 450 kDa, AKAP 450, AKAP 120-like protein, Centrosome- and Golgi-localized PKN-associated protein, CG-NAP, Protein hyperion, Protein kinase A-anchoring protein 9, PRKA9, Protein yotiao, AKAP9, AKAP350, AKAP450, KIAA0803 |
| Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AKAP9 / YOTIAO Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AKAP9 |
|---|---|
| Synonyms | AKAP350, AKAP450, KIAA0803 |
| Function | Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus (PubMed:10202149, PubMed:15047863). Required for microtubule nucleation at the cis-side of the Golgi apparatus (PubMed:15047863, PubMed:19242490). Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed:25657325). In complex with PDE4DIP isoform 13/MMG8/SMYLE, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:27666745, PubMed:28814570). In complex with PDE4DIP isoform 13/MMG8/SMYLE, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension also from the centrosome to the cell periphery (PubMed:29162697). |
| Cellular Location | Golgi apparatus. Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in parietal cells (PubMed:9915845). Recruited to the Golgi apparatus by GM130/GOLGA2 (PubMed:25657325). Localization at the centrosome versus Golgi apparatus may be cell line-dependent. In SKBr3 and HEK293F cells, exclusively located at the centrosome (PubMed:29162697). In HeLa, MDA- MB231 and RPE-1 cells, detected at the Golgi apparatus (PubMed:25217626, PubMed:29162697). In SK-BR-3 cells, recruited to the centrosome in the presence of CDK5RAP2 (PubMed:29162697) |
| Tissue Location | Widely expressed (PubMed:10202149). Isoform 4: Highly expressed in skeletal muscle and in pancreas (PubMed:9482789) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is expected to recognize isoforms 2 and 3 (NP_005742.4; NP_671714.1)
References
Association of a common AKAP9 variant with breast cancer risk: a collaborative analysis. Frank B, Wiestler M, Kropp S, Hemminki K, Spurdle AB, Sutter C, Wappenschmidt B, Chen X, Beesley J, Hopper JL, Meindl A, Kiechle M, Slanger T, Bugert P, Schmutzler RK, Bartram CR, Flesch-Janys D, Mutschelknauss E, Ashton K, Salazar R, Webb E, Hamann U, Br J. Natl. Cancer Inst. 2008 Mar 100 (6): 437-42. PMID: 18334708
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