SMC5 Antibody (Internal region)
Peptide-affinity purified goat antibody
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Application
| IF, FC, Pep-ELISA |
---|---|
Primary Accession | Q8IY18 |
Other Accession | NP_055925.2, 23137, 226026 (mouse), 293967 (rat) |
Predicted | Human, Mouse, Rat, Dog, Cow |
Host | Goat |
Clonality | Polyclonal |
Concentration | 0.5 mg/ml |
Isotype | IgG |
Calculated MW | 128806 Da |
Gene ID | 23137 |
---|---|
Other Names | Structural maintenance of chromosomes protein 5, SMC protein 5, SMC-5, hSMC5, SMC5, KIAA0594, SMC5L1 |
Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SMC5 Antibody (Internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SMC5 |
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Synonyms | KIAA0594, SMC5L1 |
Function | Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541). |
Cellular Location | Nucleus. Chromosome {ECO:0000250|UniProtKB:Q8CG46}. Nucleus, PML body. Chromosome, telomere. Note=Associates with chromatin (PubMed:25931565). Colocalizes with SMC6 on the X-Y chromosome pair within the sex vesicle during late pachytene/diplotene (By similarity) Localizes to PML nuclear bodies in ALT cell lines (PubMed:17589526) Accumulates with RAD18 and the SLF1-SLF2 complex at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565) {ECO:0000250|UniProtKB:Q8CG46, ECO:0000269|PubMed:25931565} |
Tissue Location | Widely expressed (PubMed:11408570). Strongly expressed in testis (PubMed:11408570). |
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Provided below are standard protocols that you may find useful for product applications.
References
Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability. Pebernard S, Perry JJ, Tainer JA, Boddy MN. Mol Biol Cell. 2008 Oct;19(10):4099-109. PMID: 18667531
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