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SMC5 Antibody (Internal region)

Peptide-affinity purified goat antibody

     
  • IF - SMC5 Antibody (Internal region) AF2971a
    EB09424 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing speckled nuclear and cytoplasmic staining. The nuc
    detail
  • FC - SMC5 Antibody (Internal region) AF2971a
    EB09424 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC, Pep-ELISA
Primary Accession Q8IY18
Other Accession NP_055925.2, 23137, 226026 (mouse), 293967 (rat)
Predicted Human, Mouse, Rat, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 128806 Da
Additional Information
Gene ID 23137
Other Names Structural maintenance of chromosomes protein 5, SMC protein 5, SMC-5, hSMC5, SMC5, KIAA0594, SMC5L1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMC5 Antibody (Internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMC5
Synonyms KIAA0594, SMC5L1
Function Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541).
Cellular Location Nucleus. Chromosome {ECO:0000250|UniProtKB:Q8CG46}. Nucleus, PML body. Chromosome, telomere. Note=Associates with chromatin (PubMed:25931565). Colocalizes with SMC6 on the X-Y chromosome pair within the sex vesicle during late pachytene/diplotene (By similarity) Localizes to PML nuclear bodies in ALT cell lines (PubMed:17589526) Accumulates with RAD18 and the SLF1-SLF2 complex at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565) {ECO:0000250|UniProtKB:Q8CG46, ECO:0000269|PubMed:25931565}
Tissue Location Widely expressed (PubMed:11408570). Strongly expressed in testis (PubMed:11408570).
Research Areas
Citations (0)
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References

Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability. Pebernard S, Perry JJ, Tainer JA, Boddy MN. Mol Biol Cell. 2008 Oct;19(10):4099-109. PMID: 18667531

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$ 216.00
Cat# AF2971a
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