NEBL / nebulette Antibody (internal region)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| E |
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Primary Accession | O76041 |
Other Accession | NP_998734.1, NP_001166955.1, 10529, 74103 (mouse) |
Predicted | Human, Mouse, Dog |
Host | Goat |
Clonality | Polyclonal |
Concentration | 0.5 mg/ml |
Isotype | IgG |
Calculated MW | 116453 Da |
Gene ID | 10529 |
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Other Names | Nebulette, Actin-binding Z-disk protein, NEBL, LNEBL |
Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | NEBL / nebulette Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NEBL (HGNC:16932) |
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Function | Binds to actin and plays an important role in the assembly of the Z-disk. May functionally link sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres (PubMed:27733623). Isoform 2 might play a role in the assembly of focal adhesion (PubMed:15004028). |
Cellular Location | [Isoform 2]: Cytoplasm |
Tissue Location | Abundantly expressed in cardiac muscle, but not in skeletal or smooth muscle. Localized to Z-lines in cardiac cells and to dense bodies in nonmuscle cells. Isoform 2 is expressed in non-muscle cells such as in fibroblasts |
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
References
The nebulette repeat domain is necessary for proper maintenance of tropomyosin with the cardiac sarcomere. Bonzo JR, Norris AA, Esham M, Moncman CL, Exp Cell Res. 2008 Nov 15;314(19):3519-30. Epub 2008 Sep 16. PMID: 18823973
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