|Other Accession||NP_057374.3, 51720, 20184 (mouse), 290997 (rat)|
|Predicted||Human, Mouse, Rat, Pig, Dog|
|Calculated MW||79727 Da|
|Other Names||BRCA1-A complex subunit RAP80, Receptor-associated protein 80, Retinoid X receptor-interacting protein 110, Ubiquitin interaction motif-containing protein 1, UIMC1, RAP80, RXRIP110|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAP80 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.36). Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.|
|Cellular Location||Nucleus. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs)|
|Tissue Location||Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis|
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Provided below are standard protocols that you may find useful for product applications.
Familial breast cancer screening reveals an alteration in the RAP80 UIM domain that impairs DNA damage response function. Nikkilä J, Coleman KA, Morrissey D, Pylkäs K, Erkko H, Messick TE, Karppinen SM, Amelina A, Winqvist R, Greenberg RA. Oncogene 2009 Apr 28 (16): 1843-52. PMID: 19305427
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