|Other Accession||NP_075387.1, NP_542418.1, 65110|
|Calculated MW||54696 Da|
|Other Names||Regulator of nonsense transcripts 3A, Nonsense mRNA reducing factor 3A, Up-frameshift suppressor 3 homolog A, hUpf3, UPF3A, RENT3A, UPF3|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UPF3A / RENT3A Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation.|
|Cellular Location||Nucleus. Cytoplasm. Note=Shuttling between the nucleus and the cytoplasm|
|Tissue Location||Isoform 1 is strongly expressed in testis, uterus, muscle, fetal brain and spinal cord. Isoform 2 is strongly expressed in fetal brain and spinal cord|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize both isoforms (NP_075387.1; NP_542418.1).
Mutations in UPF3B, a member of the nonsense-mediated mRNA decay complex, cause syndromic and nonsyndromic mental retardation. Tarpey PS, Raymond FL, Nguyen LS, Rodriguez J, Hackett A, Vandeleur L, Smith R, Shoubridge C, Edkins S, Stevens C, O'Meara S, Tofts C, Barthorpe S, Buck G, Cole J, Halliday K, Hills K, Jones D, Mironenko T, Perry J, Varian J, West S, Widaa S, Teague J, Di Nature genetics 2007 Sep 39 (9): 1127-33. PMID: 17704778
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