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TGFBI Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - TGFBI Antibody (internal region) AF3086a
    AF3086a (0.3 µg/ml) staining of Human Kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - TGFBI Antibody (internal region) AF3086a
    AF3086a (3.8 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • WB - TGFBI Antibody (internal region) AF3086a
    EB09795 (0.3µg/ml) staining of Human Kidney (A), Colorectal cancer (B), (0.1ug/ml) Human Prostate (C) and Rat Kidney (D) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
    detail
  • IHC - TGFBI Antibody (internal region) AF3086a
    EB09795 (3.8µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • IF - TGFBI Antibody (internal region) AF3086a
    EB09795 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing golgi and membrane staining. The nuclear stain is
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, Pep-ELISA
Primary Accession Q15582
Other Accession NP_000349.1, 7045, 21810 (mouse), 24511 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 74681 Da
Additional Information
Gene ID 7045
Other Names Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, Kerato-epithelin, RGD-containing collagen-associated protein, RGD-CAP, TGFBI, BIGH3
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTGFBI Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TGFBI
Synonyms BIGH3
Function Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity).
Cellular Location Secreted. Secreted, extracellular space, extracellular matrix Note=May be associated both with microfibrils and with the cell surface (PubMed:8077289).
Tissue Location Highly expressed in the corneal epithelium (PubMed:27609313, PubMed:8077289). Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas (PubMed:8077289)
Research Areas
Citations (0)
citation

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References

Clinical and genetic features of TGFBI-linked corneal dystrophies in Mexican population: description of novel mutations and novel genotype-phenotype correlations. Zenteno JC, Correa-Gomez V, Santacruz-Valdez C, Suarez-Sanchez R, Villanueva-Mendoza C, Experimental eye research 2009 Aug 89 (2): 172-7. PMID: 19303004

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$ 341.00
Cat# AF3086a
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