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CHCHD3 (aa151-164) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - CHCHD3 (aa151-164) Antibody (internal region) AF3115a
    AF3115a (0.3 µg/ml) staining of Human Placenta lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • IHC - CHCHD3 (aa151-164) Antibody (internal region) AF3115a
    AF3115a (5 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9NX63
Other Accession NP_060282.1, 54927, 66075 (mouse), 296966 (rat)
Reactivity Human
Predicted Mouse, Rat, Dog, Sheep, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 26152 Da
Additional Information
Gene ID 54927
Other Names MICOS complex subunit MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, CHCHD3, MIC19, MINOS3
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHCHD3 (aa151-164) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHCHD3
Synonyms MIC19, MINOS3
Function Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:25781180).
Cellular Location Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q9CRB9}; Lipid-anchor; Intermembrane side {ECO:0000250|UniProtKB:Q9CRB9}. Cytoplasm. Nucleus Mitochondrion
Tissue Location Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue.
Research Areas
Citations (0)
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References

The mitochondrial inner membrane protein mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix.
Coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11 Xie,J., Marusich,M.F., Souda,P., Whitelegge,J. and Capaldi,R.A. FEBS Lett. 581 (18), 3545-3549 (2007) PMID: 17624330

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$ 341.00
Cat# AF3115a
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