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E2F3 (mouse) Antibody (C-Term)

Peptide-affinity purified goat antibody

     
  • WB - E2F3 (mouse) Antibody (C-Term) AF3164a
    AF3164a (1 µg/ml) staining of Mouse fetal Lung lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O00716
Other Accession NP_034223.1, 1871, 13557 (mouse)
Reactivity Mouse
Predicted Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 49162 Da
Additional Information
Gene ID 1871
Other Names Transcription factor E2F3, E2F-3, E2F3, KIAA0075
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsE2F3 (mouse) Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name E2F3
Synonyms KIAA0075
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)

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References

The E2F1-3 transcription factors are essential for cellular proliferation. Wu L, Timmers C, Maiti B, Saavedra HI, Sang L, Chong GT, Nuckolls F, Giangrande P, Wright FA, Field SJ, Greenberg ME, Orkin S, Nevins JR, Robinson ML, Leone G, Nature 2001 Nov 414 (6862): 457-62. PMID: 11719808

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$ 335.00
Cat# AF3164a
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