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E2F4 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - E2F4 Antibody (internal region) AF3165a
    AF3165a (2 µg/ml) staining of Human Brain (Cerebellum) lysate (35 µg protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q16254
Other Accession NP_001941.2, 1874, 104394 (mouse)
Reactivity Human
Predicted Mouse, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 43960 Da
Additional Information
Gene ID 1874
Other Names Transcription factor E2F4, E2F-4, E2F4
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsE2F4 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name E2F4
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Cellular Location Nucleus.
Tissue Location Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)

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References

Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations. Lu Y, Dollé ME, Imholz S, van 't Slot R, Verschuren WM, Wijmenga C, Feskens EJ, Boer JM, Journal of lipid research 2008 Dec 49 (12): 2582-9. PMID: 18660489

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$ 325.00
Cat# AF3165a
Size:
Quantity:
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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