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Herculin / MYF6 Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - Herculin / MYF6 Antibody (internal region) AF3172a
    HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MYF6 with MYC tag probed with AF3172a (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AF3172a (0.5mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P23409
Other Accession NP_002460.1, 4618, 17878 (mouse), 25714 (rat)
Reactivity Human
Predicted Mouse, Rat, Pig, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 26953 Da
Additional Information
Gene ID 4618
Other Names Myogenic factor 6, Myf-6, Class C basic helix-loop-helix protein 4, bHLHc4, Muscle-specific regulatory factor 4, MYF6, BHLHC4, MRF4
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHerculin / MYF6 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYF6
Synonyms BHLHC4, MRF4
Function Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Cellular Location Nucleus.
Tissue Location Skeletal muscle.
Research Areas
Citations (0)
citation

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References

The myogenic basic helix-loop-helix family of transcription factors shows similar requirements for SWI/SNF chromatin remodeling enzymes during muscle differentiation in culture. Roy K, de la Serna IL, Imbalzano AN, The Journal of biological chemistry 2002 Sep 277 (37): 33818-24. PMID: 12105204

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$ 341.00
Cat# AF3172a
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