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BLM Antibody (C-Term)

Peptide-affinity purified goat antibody

  • WB - BLM Antibody (C-Term) AF3188a
    AF3188a (0.03 µg/ml) staining of nuclear HeLa lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54132
Other Accession NP_000048.1, 641, 113959 (rat)
Reactivity Human
Predicted Rat
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 159000 Da
Additional Information
Gene ID 641
Other Names Bloom syndrome protein,, DNA helicase, RecQ-like type 2, RecQ2, RecQ protein-like 3, BLM, RECQ2, RECQL3
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBLM Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BLM
Synonyms RECQ2, RECQL3
Function Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).
Cellular Location Nucleus. Note=Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner
Research Areas
Citations (0)

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Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. Hosgood HD, Zhang L, Shen M, Berndt SI, Vermeulen R, Li G, Yin S, Yeager M, Yuenger J, Rothman N, Chanock S, Smith M, Lan Q, Occupational and environmental medicine 2009 Dec 66 (12): 848-53. PMID: 19773279

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$ 325.00
Cat# AF3188a
(40 western blots)
Availability: 7-10 days
Bulk Size
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