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POU3F2 / BRN2 / OCT7 Antibody (internal region, near N-Term)
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS
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Application 
| WB, E |
|---|---|
| Primary Accession | P20265 |
| Other Accession | NP_005595.2, 5454, 18992 (mouse), 29588 (rat) |
| Reactivity | Human, Mouse, Rat |
| Predicted | Pig, Cow |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/ml |
| Isotype | IgG |
| Calculated MW | 46893 Da |
| Gene ID | 5454 |
|---|---|
| Other Names | POU domain, class 3, transcription factor 2, Brain-specific homeobox/POU domain protein 2, Brain-2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Oct-7, Octamer-binding transcription factor 7, OTF-7, POU3F2, BRN2, OCT7, OTF7 |
| Format | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POU3F2 / BRN2 / OCT7 Antibody (internal region, near N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POU3F2 |
|---|---|
| Synonyms | BRN2, OCT7, OTF7 |
| Function | Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity). |
| Cellular Location | Nucleus. |
| Tissue Location | Expressed specifically in the neuroectodermal cell lineage |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
References
Expression and purification of human full-length N Oct-3, a transcription factor involved in melanoma growth. Cabos-Siguier B, Steunou AL, Joseph G, Alazard R, Ducoux-Petit M, Nieto L, Monsarrat B, Erard M, Clottes E, Protein expression and purification 2009 Mar 64 (1): 39-46. PMID: 18996486
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