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PBX1 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - PBX1 Antibody (internal region) AF3213a
    AF3213a (0.5 µg/ml) staining of K562 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • WB - PBX1 Antibody (internal region) AF3213a
    AF3213a (0.5 µg/ml) staining of Mouse Eye lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P40424
Other Accession NP_002576.1, 5087, 18514 (mouse)
Reactivity Human, Mouse, Rat
Predicted Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 46626 Da
Additional Information
Gene ID 5087
Other Names Pre-B-cell leukemia transcription factor 1, Homeobox protein PBX1, Homeobox protein PRL, PBX1, PRL
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPBX1 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PBX1
Synonyms PRL
Function Binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Probably in complex with MEIS2, is involved in transcriptional regulation by KLF4. Acts as a transcriptional activator of NKX2-5 and a transcriptional repressor of CDKN2B. Together with NKX2-5, it is required for spleen development through a mechanism that involves CDKN2B repression (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108}
Tissue Location Expressed in all tissues except in cells of the B and T lineage
Research Areas
Citations (0)

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Gfi1 integrates progenitor versus granulocytic transcriptional programming. Horman SR, Velu CS, Chaubey A, Bourdeau T, Zhu J, Paul WE, Gebelein B, Grimes HL. Blood 2009 May 113 (22): 5466-75. PMID: 19346496

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$ 325.00
Cat# AF3213a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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