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RFX5 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - RFX5 Antibody (internal region) AF3226a
    AF3226a (2 µg/ml) staining of Daudi lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • WB - RFX5 Antibody (internal region) AF3226a
    AF3226a (2 µg/ml) staining of NIH3T3 nuclear lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48382
Other Accession NP_000440.1, 5993, 53970 (mouse), 310659 (rat)
Reactivity Human, Mouse
Predicted Rat, Rabbit, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 65323 Da
Additional Information
Gene ID 5993
Other Names DNA-binding protein RFX5, Regulatory factor X 5, RFX5
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRFX5 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RFX5
Function Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
Cellular Location Nucleus.
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Reported variants represent identical protein: NP_000440.1, NP_001020774.1


HDAC2 deacetylates class II transactivator and suppresses its activity in macrophages and smooth muscle cells. Kong X, Fang M, Li P, Fang F, Xu Y, Journal of molecular and cellular cardiology 2009 Mar 46 (3): 292-9. PMID: 19041327

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$ 325.00
Cat# AF3226a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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