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PCBP1 (aa223-234) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - PCBP1 (aa223-234) Antibody (internal region) AF3383a
    AF3383a (0.1 µg/ml) staining of PBMC lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
  • WB - PCBP1 (aa223-234) Antibody (internal region) AF3383a
    AF3383a (0.1 µg/ml) staining of Rat Spleen (A) and Thymus (B) lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q15365
Other Accession NP_006187.2, 5093, 23983 (mouse), 500242 (rat)
Reactivity Human, Rat
Predicted Mouse, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 37498 Da
Additional Information
Gene ID 5093
Other Names Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, hnRNP E1, Nucleic acid-binding protein SUB2.3, PCBP1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCBP1 (aa223-234) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCBP1 {ECO:0000303|PubMed:7607214, ECO:0000312|HGNC:HGNC:8647}
Function Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed:7607214, PubMed:7556077, PubMed:8152927, PubMed:15731341). Together with PCBP2, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity).
Cellular Location Nucleus. Cytoplasm. Note=Loosely bound in the nucleus (PubMed:7607214). May shuttle between the nucleus and the cytoplasm (PubMed:7607214).
Tissue Location Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
Research Areas
Citations (0)
citation

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References

Depletion of the poly(C)-binding proteins alphaCP1 and alphaCP2 from K562 cells leads to p53-independent induction of cyclin-dependent kinase inhibitor (CDKN1A) and G1 arrest. Waggoner SA, Johannes GJ, Liebhaber SA, The Journal of biological chemistry 2009 Apr 284 (14): 9039-49. PMID: 19211566

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$ 341.00
Cat# AF3383a
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