|Application ||WB, E|
|Other Accession||NP_078781.3, NP_001171708.1, NP_001171709.1, 79400|
|Calculated MW||86439 Da|
|Other Names||NADPH oxidase 5, 1.6.3.-, NOX5|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NOX5 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin.|
|Cellular Location||Membrane; Multi-pass membrane protein Isoform v5: Endoplasmic reticulum.|
|Tissue Location||Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1).
Sphingosylphosphorylcholine down-regulates filaggrin gene transcription through NOX5-based NADPH oxidase and cyclooxygenase-2 in human keratinocytes. Choi H, Kim S, Kim HJ, Kim KM, Lee CH, Shin JH, Noh M, Biochemical pharmacology 2010 Jul 80 (1): 95-103. PMID: 20230798
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