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ODF2 Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - ODF2 Antibody (internal region) AF3425a
    AF3425a (3 µg/ml) staining of C57/B6 mouse CTL lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Everest grant winner Dr. J. Griffiths, University of Cambridge, UK
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5BJF6
Other Accession NP_002531.3, NP_702915.1, 4957, 18286 (mouse), 29479 (rat)
Reactivity Mouse
Predicted Human, Rat, Pig, Dog
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 95401 Da
Additional Information
Gene ID 4957
Other Names Outer dense fiber protein 2, Cenexin, Outer dense fiber of sperm tails protein 2, ODF2
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsODF2 Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ODF2
Function Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, spindle pole. Note=Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles
Tissue Location Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.
Research Areas
Citations (0)

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This antibody is expected to recognize reported isoform 1 (NP_002531.3).


Plk1-dependent and -independent roles of an ODF2 splice variant, hCenexin1, at the centrosome of somatic cells. Soung NK, Park JE, Yu LR, Lee KH, Lee JM, Bang JK, Veenstra TD, Rhee K, Lee KS, Developmental cell 2009 Apr 16 (4): 539-50. PMID: 19386263

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$ 325.00
Cat# AF3425a
(40 western blots)
Availability: 7-10 days
Bulk Size
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