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CYB5R3 / Dia 1 Antibody (C-Term)

Peptide-affinity purified goat antibody

  • WB - CYB5R3 / Dia 1  Antibody (C-Term) AF3454a
    AF3454a (0.01 µg/ml) staining of Human Umbilical Cord lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • IHC - CYB5R3 / Dia 1  Antibody (C-Term) AF3454a
    AF3454a (3.8 µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00387
Other Accession NP_000389.1, NP_001123291.1, NP_001165131.1, 1727
Reactivity Human
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 34235 Da
Additional Information
Gene ID 1727
Other Names NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase,, Diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, CYB5R3, DIA1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYB5R3 / Dia 1 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYB5R3
Synonyms DIA1
Function Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
Cellular Location Isoform 1: Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Mitochondrion outer membrane; Lipid-anchor; Cytoplasmic side
Tissue Location Isoform 2 is expressed at late stages of erythroid maturation
Research Areas
Citations (0)

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This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1).


RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1. Fernandez-Borja M, Janssen L, Verwoerd D, Hordijk P, Neefjes J, J. Cell. Sci. 2005 Jun 118 (Pt 12): 2661-70. PMID: 15944396

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$ 325.00
Cat# AF3454a
(40 western blots)
Availability: 7-10 days
Bulk Size
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