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PRDM14 (aa533-544) Antibody (internal region)

Peptide-affinity purified goat antibody

  • WB - PRDM14 (aa533-544) Antibody (internal region) AF3481a
    AF3481a (2 µg/ml) staining of A549 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9GZV8
Other Accession NP_078780.1, 63978, 383491 (mouse), 100365184 (rat)
Reactivity Human
Predicted Mouse, Rat, Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 64062 Da
Additional Information
Gene ID 63978
Other Names PR domain zinc finger protein 14, 2.1.1.-, PR domain-containing protein 14, PRDM14
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRDM14 (aa533-544) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRDM14
Function Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.
Cellular Location Nucleus.
Tissue Location Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level)
Research Areas
Citations (0)

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A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. Chia NY, Chan YS, Feng B, Lu X, Orlov YL, Moreau D, Kumar P, Yang L, Jiang J, Lau MS, Huss M, Soh BS, Kraus P, Li P, Lufkin T, Lim B, Clarke ND, Bard F, Ng HH, Nature. 2010 Nov 11;468(7321):316-20. PMID: 20953172

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$ 325.00
Cat# AF3481a
(40 western blots)
Availability: 7-10 days
Bulk Size
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