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NQO1 (isoform a) Antibody (internal region)

Peptide-affinity purified goat antibody

     
  • WB - NQO1 (isoform a) Antibody (internal region) AF3483a
    AF3483a (0.3 µg/ml) staining of Human Kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P15559
Other Accession NP_000894.1, 1728
Reactivity Human
Predicted Pig, Dog, Cow
Host Goat
Clonality Polyclonal
Concentration 0.5 mg/ml
Isotype IgG
Calculated MW 30868 Da
Additional Information
Gene ID 1728
Other Names NAD(P)H dehydrogenase [quinone] 1, 1.6.5.2, Azoreductase, DT-diaphorase, DTD, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, QR1, NQO1, DIA4, NMOR1
Format 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNQO1 (isoform a) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NQO1 {ECO:0000303|PubMed:1657151, ECO:0000312|HGNC:HGNC:2874}
Function Flavin-containing quinone reductase that catalyzes two- electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (PubMed:8999809, PubMed:9271353) (By similarity). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion (PubMed:8999809, PubMed:9271353, PubMed:15102952). Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential (PubMed:8999809). Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome (PubMed:15687255, PubMed:28291250).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:P05982}
Research Areas
Citations (0)
citation

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Background

This antibody is expected to recognize reported isoform a (NP_000894.1) only.

References

NQO1 stabilizes p53 through a distinct pathway. Asher G, Lotem J, Kama R, Sachs L, Shaul Y, Proceedings of the National Academy of Sciences of the United States of America 2002 Mar 99 (5): 3099-104. PMID: 11867746

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$ 341.00
Cat# AF3483a
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Availability: 7-10 days
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