|Application ||WB, E|
|Other Accession||NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1, 1641, 13193 (mouse), 84394 (rat)|
|Predicted||Human, Rat, Pig, Dog, Cow|
|Calculated MW||49318 Da|
|Other Names||Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DCX, DBCN, LISX|
|Format||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Doublecortin / DCX (aa232-242) Antibody (internal region) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.|
|Cellular Location||Cytoplasm. Cell projection. Note=Localizes at neurite tips.|
|Tissue Location||Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
MiR-128 up-regulation inhibits Reelin and DCX expression and reduces neuroblastoma cell motility and invasiveness. Evangelisti C, Florian MC, Massimi I, Dominici C, Giannini G, Galardi S, BuÃ¨ MC, Massalini S, McDowell HP, Messi E, Gulino A, Farace MG, CiafrÃ¨ SA, The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Dec 23 (12): 4276-87. PMID: 19713529
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